Absorbance - material captures the energy of a light wave, Refraction - the fact that light waves change direction as they enter a new medium, Magnification - the ability of a lens to enlarge the image of an object when compared to the real object, Resolution - the ability to tell that 2 separate points are separate, Robert Hooke - Used his compound microscope to view dead cork cells, Light Microscope - Microscopes that use visible light to observe specimens, Brightfield Microscope - Sample is dark while the background is bright, Darkfield Microscope - Sample is bright while the background is dark, Oil Immersion - a technique used to increase the resolving power of a microscope, Electron Microscope - microscope that uses magnets to focus electron beams similarly to the way that light microscopes use lenses to focus light, TEM - uses electron beams that pass through a specimen to visualize small images, SEM - uses electron beams to visualize surfaces, Heat Fixing - ensures the elimination of contaminating organisms from the smear preparation by a heat source, Negative Stains - stains background, not the specimen, Acidic Stains - stain positively charged molecules and structures such as proteins, Basic Stains - stain negatively charged molecules and structures such as nucleic acids and proteins, Reflection - wave bounces off of a material, Transmission - wave travels through a material, Antonie Van Leeuenhoek - Credited as being the first person to observe microbes, including bacteria, which he called “animicaules” and “wee little beasties”, Robert Hooke - used his compounds microscope to view cork cells,

BIOL 20 Session Ch. 2

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