1) Which of the following methods brings about cell lysis due to cavitation induced by rapid localized pressure changes? a) microwaving b) B. gamma irradiation c) C. ultraviolet radiation d) D. sonication 2) What organism is associated with BSL-2? a) Staph. aureus b) Mycobacterium tuberculosis c) E. bola 3) What organism associate with BSL-3? a) Mycobacterium tuberculosis b) E. coli c) Staph. aureus  4) Which of the following terms is used to describe the time required to kill all of the microbes within a sample at a given temperature? a) D-value b) B. thermal death point c) C. thermal death time d) D. decimal reduction time 5) Which of the following microbial control methods does not actually kill microbes or inhibit their growth but instead removes them physically from samples? a) filtration b) B. desiccation c) C. lyophilization d) D. nonionizing radiation 6) Ionizing radiation can penetrate surfaces, but nonionizing radiation cannot. a) True b) False 7) T/F - Moist-heat sterilization protocols require the use of higher temperatures for longer periods of time than do dry-heat sterilization protocols do. a) False b) True  8) HEPA filters are required for which Biological Safety Level (BSL)? a) BSL 1 b) B. BSL 2 c) C. BSL 3 d) D. BSL 4 9) Which of the following chemicals can be described as salts of fatty acids capable of emulsifying fats and lipids and used extensively for degerming? a) chlorohexidine b) B. phenols c) C. soaps d) D. hydrogen peroxide 10) The biosafety level (BSL) for a clinical microbiology laboratory working with indigenous and exotic potentially airborne pathogens, such as tuberculosis bacteria, is: a) BSL 1 b) B. BSL 2 c) C. BSL 3 d) D. BSL 4 11) Which concentration of ethanol/alcohol is the most effective to kill microbes? a) A. 100 percent b) B. 70 percent c) C. 50 percent d) D. 40 percent e) E. 30 percent 12) T/F - Antiseptics are chemicals used to clean and inhibit microorganism growth on nonliving surfaces. a) True b) False 13) What are fomites?  a) inanimate items, such as doorknobs, toys, or towels, which may harbor microbes and aid in disease transmission b) Animate objects such as a couch or a vector, 14) What factors determine the BSL? a) E. coli b) B. subtilis c) agents that do not cause infection in healthy humans d) Tubercluois e) E. Bola  15) What types of agents are associated with BSL2? a) S. aureus b) Salmonella spp c) hepatitis, d) mumps e) E.coli 16) What types of agents are associated with BSL3? a) M. tuberculosis b) B. anthracis, c) West Nile Viru d) smallpox virus 17) What types of agents are assiocated with BSL 4  a) Ebola Virus b) Marburg Virus c) B. anthracis d) West Nile

Biol chapter 13

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