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1) What happens to DNA when exposed to UV light? a) It undergoes denaturation b) It forms thymine dimers c) It undergoes condensation d) It breaks into nucleotides 2) Which test do we use hydrogen peroxide for? a) Amylase b) Catalase c) Urease d) Oxidase 3) What is the difference between the different sugar fermentation tubes? a) The type of indicator b) The type of sugar used c) The presence of a Durham Tube d) The type of bacteria used 4) Urease breaks down what waste product? a) Carbon Dioxide b) Hydrogen c) Water d) Ammonia 5) (T/F) Bile Salts and Crystal Violet are incorporated into the Mac Conkey agar plate (MAC) are gram negative inhibitors a) True b) False 6) What occurs in the Completed Test in MPN testing? a) Metallic sheen on special agar indicating presence of E. coli b) Testing for the production of gas in a Durham tube c) pH color change of the broth d) Determining the presence of specific enzymes 7) Which test can detect glucose fermenters which produce non-acidic or acetoin? a) MR test b) VP test c) Indole test d) Nitrate test 8) What protects bacterial cells from UV light damage? a) Their rod shapes b) Being gram + c) Creating endospores d) Creating capsules 9) What does the yellow halo around the starch plate test indicate? a) Iodine has been hydrolyzed b) No starch hydrolysis c) Ammonia has been hydrolyzed d) Starch has been hydrolyzed 10) Which of the following is not a characteristic of coliform bacteria? a) Cocci  b) Gram Negative c) Ferments lactose d) Produces gas 11) Positive EMB test shows: a) gram negative coliform b) gram negative non coliform 12) Urease test shows that the pH indicator phenol red will only turn pink if the organism can hydrolyse urea to which of the following a) Ammonia b) Nitrate c) Nitric Oxide d) Nitrite 13) In a cell, urea is a waste product of what type of digestion? a) Protein b) Carbohydrate c) Fat d) Cellular 14) Which of the following UV rays is biocidal? a) UVA b) UVB c) UVC d) Infrared 15) Which of the following describes a bacteria with multiple flagella all around a) lophotrichous b) monotrichous c) peritrichous d) amphitrichous 16) The mannitol salt platMannitol Salt agar plate is used to distinguish species of which bacteria?e  a) Staphylococci b) Streptococci c) Enterococci d) Neisseria 17) What does the yellow halo around the starch plate test indicate? a) Iodine has been hydrolyzed b) No starch hydrolysis c) Ammonia has been hydrolyzed d) Starch has been hydrolyzed 18) X rays and Gamma rays are an example of what a) Ionizing Radiation b) Non-Ionizing Radiation 19) Which of the following sugars is a monosaccharide? a) LactoseCocci  b) Sucrose c) Glucose d) Maltose 20) What is the reagent used to test for any presence of starch? a) Hydrogen Peroxide b) Iodine c) Benedict’s Solution d) Barium Chloride 21) Which pigment indicates the motility of bacteria when TTC is incorporated into the moving bacterial cell? a) TTC b) Formaza c) Phenol Red d) Methyl Red 22) How is a positive result for starch hydrolysis indicated in starch plates? a) Presence of a dark brown/purple coloration around the culture b) Clear halo around the culture c) Formation of greenish pigments d) Complete inhibition of bacterial growth 23) Which enzyme is being tested for starch hydrolysis? a) Amylase b) Lipase c) Casease d) Gelatinase 24) What is the purpose of the oxidase test in microbiology? a) To detect the presence of gas b) To identify carbohydrate fermentation c) To determine the presence of cytochrome c d) To differentiate between gram-positive and gram-negative bacteria 25) What enzyme catalyzes the breakdown of lipids? a) Protease b) Cellulase c) Lipase d) Amylase 26) Which enzyme is responsible for breaking down starch? a) Amylase b) Lipase c) Protease d) Gelatinase 27) How can the fermentation of carbohydrates be tested? a) Using a spectrophotometer b) By measuring electrical conductivity c) With fermentation broths containing phenol red and Durham tubes d) By observing bacterial growth under a microscope 28) How is the production of gas detected in fermentation testing? a) By observing color change in the phenol red b) By measuring the temperature change c) By observing bubbles in the Durham tube d) By conducting a gas chromatography analysis 29) What is the purpose of phenol red in fermentation testing? a) To measure the concentration of carbohydrates b) To detect the presence of gas c) To indicate a change in pH d) To facilitate bacterial growth 30) Which of the following shows a positive result from the Confirmatory Test a) Gram positive coliforms b) Gram negative coliforms

Practice Lab 2 Exam KAHOOT 5.2.24

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