Make E.coli cells competent by adding calcium chloride., Label the Tubes P+ and P-, Place E.coli into each microtube., Add plasmid into P+ microtube., Place labeled tubes on ice for 15 minutes., Heath Shock Cells in Warm Water Bath (45 sec.), Place tubes on ice for 3-5 minutes, Resuspend the cells and plate cells onto each plate., Incubate cells., Rest Cells..

Bacterial Transformation

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